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Notch signaling pathway is required for Ajuba (JUB)-induced chemoresistance. (a) Protein levels of JUB and Notch1 (NICD) in the indicated cells. (b) Colony number in the indicated groups. (c) Sphere number in the indicated groups. Data are presented as the mean ± standard deviation SD values of three independent experiments; n = 3, ** P < 0.01, *** P < 0.001. (GADPH: Glyceraldehyde-3-phosphate dehydrogenase, JUB: Ajuba, DAPT: Notch signaling inhibitor <t>gamma-secretase</t> inhibitor, 5-FU: 5-Fluorouracil)
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Notch signaling pathway is required for Ajuba (JUB)-induced chemoresistance. (a) Protein levels of JUB and Notch1 (NICD) in the indicated cells. (b) Colony number in the indicated groups. (c) Sphere number in the indicated groups. Data are presented as the mean ± standard deviation SD values of three independent experiments; n = 3, ** P < 0.01, *** P < 0.001. (GADPH: Glyceraldehyde-3-phosphate dehydrogenase, JUB: Ajuba, DAPT: Notch signaling inhibitor <t>gamma-secretase</t> inhibitor, 5-FU: 5-Fluorouracil)
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Notch signaling pathway is required for Ajuba (JUB)-induced chemoresistance. (a) Protein levels of JUB and Notch1 (NICD) in the indicated cells. (b) Colony number in the indicated groups. (c) Sphere number in the indicated groups. Data are presented as the mean ± standard deviation SD values of three independent experiments; n = 3, ** P < 0.01, *** P < 0.001. (GADPH: Glyceraldehyde-3-phosphate dehydrogenase, JUB: Ajuba, DAPT: Notch signaling inhibitor gamma-secretase inhibitor, 5-FU: 5-Fluorouracil)

Journal: CytoJournal

Article Title: Targeting the Ajuba/Notch axis increases the sensitivity of colon cancer cells to 5-fluorouracil

doi: 10.25259/Cytojournal_44_2024

Figure Lengend Snippet: Notch signaling pathway is required for Ajuba (JUB)-induced chemoresistance. (a) Protein levels of JUB and Notch1 (NICD) in the indicated cells. (b) Colony number in the indicated groups. (c) Sphere number in the indicated groups. Data are presented as the mean ± standard deviation SD values of three independent experiments; n = 3, ** P < 0.01, *** P < 0.001. (GADPH: Glyceraldehyde-3-phosphate dehydrogenase, JUB: Ajuba, DAPT: Notch signaling inhibitor gamma-secretase inhibitor, 5-FU: 5-Fluorouracil)

Article Snippet: The cells were first treated with the Notch signaling inhibitor gamma-secretase inhibitor (DAPT, 10uM, Selleck, S2215), which could remarkably reduce the expression of Notch1 but did not affect the protein level of JUB [ ].

Techniques: Standard Deviation